{"id":9431,"date":"2020-10-20T17:34:41","date_gmt":"2020-10-20T17:34:41","guid":{"rendered":"http:\/\/localhost\/alquera\/?page_id=9431"},"modified":"2022-12-02T06:14:31","modified_gmt":"2022-12-02T06:14:31","slug":"fluorescencia","status":"publish","type":"page","link":"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/fluorescencia\/","title":{"rendered":"Fluorescence - What it is and How it Works"},"content":{"rendered":"<p>Fluorescence is the phenomenon whereby some products or substances emit energy in the form of light when irradiated with ultraviolet light. Fluorescence should not be confused with phosphorescence. In phosphorescence, the particles store the energy and then radiate it after the ultraviolet light irradiation has been completed.<\/p>\n\n\n\n<p>To really understand the fluorescence process, it is necessary to remember the atomic orbital and molecular orbital model. According to the uncertainty principle, an electron can be found within a certain energy level range, in a lower or higher level. There are low-energy and high-energy orbitals. Electrons by default prefer to occupy low-energy orbitals, but can transiently occupy higher energy levels. <\/p>\n\n\n\n<p>These orbital jumps are achieved in certain substances by irradiating them with energy. These jumps are known as electronic transitions. Each type of atom has very characteristic absorption lines and emission lines.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Fluorescence applications<\/h2>\n\n\n\n<p>The phenomenon of fluorescence has many applications:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><strong>In Lighting<\/strong>Fluorescent tube: The operation of the fluorescent tube, so ubiquitous in homes and offices, depends on fluorescence. Inside the glass tube there is a partial vacuum and a small amount of mercury. An electrical discharge in the tube causes the mercury atoms to emit light. The light emitted is in the ultraviolet (UV) range, and is therefore invisible to our eyes; but the tube is coated with a layer of a fluorescent material called phosphor, which absorbs ultraviolet light and re-emits it in the visible spectrum.<\/li><li><strong>Analytical chemistry<\/strong> for laboratory techniques for the detection<\/li><li><strong>Biochemistry and medicine<\/strong>Biomolecules can be labelled with a fluorescent chemical group (fluorochrome) by a simple chemical reaction, allowing sensitive and quantitative detection of the molecule.<\/li><li><strong>Gemology<\/strong>mineralogy, mineralogy, geology and forensic sciences<\/li><li>Another application of this phenomenon is in the differentiation of <strong>counterfeit notes<\/strong> The printing of these is done with fluorescent ink, which is visible in ultraviolet light.<\/li><li>Fluorescence water assessment in hydrology.<\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Fluorescent materials. Fluorescent Stones and Minerals<\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li>An example of such substances is the <a href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/\">fluorescein sodium<\/a>a compound belonging to the xanthine family. You can see in the experiment section, in the experiment, making fluorescent water, how diluted in water it has a yellow colour. When it is in solutions of pH greater than 7 (for example by adding a few drops of ammonia to the water), it turns green, and its fluorescence increases.<\/li><\/ul>\n\n\n\n<p><a href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/fluorescencia\/fluorescencia2\/\" rel=\"attachment wp-att-9681\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-9681 aligncenter\" src=\"https:\/\/www.alquera.com\/wp-content\/uploads\/2020\/10\/fluorescencia2-300x171.png\" alt=\"\" width=\"300\" height=\"171\" srcset=\"https:\/\/www.alquera.com\/wp-content\/uploads\/2020\/10\/fluorescencia2-300x171.png 300w, https:\/\/www.alquera.com\/wp-content\/uploads\/2020\/10\/fluorescencia2.png 504w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><strong>Precious stones:<\/strong> They have fluorspar in their crystalline structures if they are not too pure.\n<ul>\n<li>Amber: They show orange-coloured radiation when receiving short-wave ultraviolet light.<\/li>\n<li>Hope diamonds and rubies: They exhibit red radiation when irradiated with short-wave ultraviolet radiation.<\/li>\n<\/ul>\n<\/li><li><strong>Minerals:<\/strong> In many cases it is due to the fact that fluorite or a derivative of fluorite is present. In this picture you can see some fluorescent minerals, the main ones being the following:<\/li><\/ul>\n\n\n\n\n\n<ul class=\"wp-block-list\"><li>Fluorite: It is the basis for most of the fluorescent elements, it presents different colour variations in the radiations, such as green, blue or pink.<\/li><li>Calcite: Similar properties to fluorite, hence its properties. It tends to emit light in pink colours.<\/li><li>Uranium: This mineral has a greenish radiation due to the fact that it is a radioactive material.<\/li><li><strong>Organic materials:<\/strong> They mainly owe their fluorescence to the mineral compounds that form them together with organic matter. The main examples are:\n<ul>\n<li>Mycena Lux-coeli fungus. It is believed that they acquire this property to attract insects to carry out spore dispersal.<\/li>\n<li>Chlorophyll: The chlorophyll present in the leaves of plants takes the sun's energy to carry out photosynthesis, and part of this energy must be dissipated in order not to damage the leaf. Part is dissipated as heat and part in the form of fluorescence. It is usually green in colour.<\/li>\n<\/ul>\n<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Fluorescence in sodium fluorescein<\/h2>\n\n\n\n<p>Sodium fluorescein is one of the substances, within the chemical products, which have the property of fluorescence and are among the most widely used, given their ease of transport and their stability. Sodium fluorescein is used both for medical purposes for ocular detection and in the hydrological field for the detection of leaks and traces in aquatic areas.<\/p>\n\n\n\n<p><p>The emission of sodium fluorescein is highest at pH 8 and at a wavelength of around 550nm. In this image you can see the emission-excitation graph of sodium fluorescein.<\/p>\n<p><iframe loading=\"lazy\" title=\"How to make a fluorescent liquid - Alquera\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/zsq1paqbVls?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p><\/p>\n\n\n\n<h3 class=\"widget-title\">Buy <\/h3><div><div class=\"woocommerce\"><ul class=\"products columns-4\">\n<li class=\"desktop-align-left tablet-align-left mobile-align-left ast-product-gallery-layout-horizontal-slider ast-magnify-disabled ast-product-single-variable ast-product-tabs-layout-horizontal ast-qv-on-image product type-product post-495 status-publish first instock product_cat-productos-experimentos product_cat-productos has-post-thumbnail taxable shipping-taxable purchasable product-type-variable has-default-attributes\">\n\t<a href=\"https:\/\/www.alquera.com\/en\/permanganato-de-potasio\/\" class=\"woocommerce-LoopProduct-link woocommerce-loop-product__link\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.alquera.com\/wp-content\/uploads\/2016\/01\/permanganato-200g-foto-nueva-300x300.png\" class=\"attachment-woocommerce_thumbnail size-woocommerce_thumbnail\" alt=\"permanganato 200g\" srcset=\"https:\/\/www.alquera.com\/wp-content\/uploads\/2016\/01\/permanganato-200g-foto-nueva-300x300.png 300w, https:\/\/www.alquera.com\/wp-content\/uploads\/2016\/01\/permanganato-200g-foto-nueva-150x150.png 150w, https:\/\/www.alquera.com\/wp-content\/uploads\/2016\/01\/permanganato-200g-foto-nueva-12x12.png 12w, https:\/\/www.alquera.com\/wp-content\/uploads\/2016\/01\/permanganato-200g-foto-nueva-100x100.png 100w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><h2 class=\"woocommerce-loop-product__title\">Potassium Permanganate<\/h2><div class=\"review-rating\"><div class=\"star-rating\"><span style=\"width:100%\" data-no-translation=\"\" data-trp-gettext=\"\">Rated <strong class=\"rating\">5.00<\/strong> out of 5<\/span><\/div><\/div>\n<\/a><a href=\"https:\/\/www.alquera.com\/en\/permanganato-de-potasio\/\" aria-describedby=\"woocommerce_loop_add_to_cart_link_describedby_495\" data-quantity=\"1\" class=\"button product_type_variable add_to_cart_button\" data-product_id=\"495\" data-product_sku=\"AL100111\" aria-label=\"Select options for \u201cPermanganato de Potasio\u201d\" rel=\"nofollow\" data-no-translation=\"\" data-trp-gettext=\"\" data-no-translation-aria-label=\"\">Select options<\/a>\t<span id=\"woocommerce_loop_add_to_cart_link_describedby_495\" class=\"screen-reader-text\">\n\t\tThis product has multiple variants. The options may be chosen on the product page\t<\/span>\n<\/li>\n<\/ul>\n<\/div><\/div>\n\n\n\n<p class=\"widget-title\">Read more<\/p><a href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/\">Sodium Fluorescein<\/a>\n<!-- Page-list plugin v.5.9 wordpress.org\/plugins\/page-list\/ -->\n<ul class=\"page-list siblings-page-list\">\n<li class=\"page_item page-item-9431 menu-item\"><a href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/fluorescencia\/\" class=\"menu-link\">Fluorescence - What it is and How it Works<\/a><\/li>\n<li class=\"page_item page-item-9429 menu-item\"><a href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/hacer-liquido-fluorescente\/\" class=\"menu-link\">Making fluorescent liquid<\/a><\/li>\n<li class=\"page_item page-item-9424 menu-item\"><a href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/usos-fluoresceina-sodica\/\" class=\"menu-link\">Uses of Fluorescein Sodium<\/a><\/li>\n\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>La fluorescencia es el fen\u00f3meno mediante el cual algunos productos o substancias emiten energ\u00eda en forma de luz una vez se les irradia con luz ultravioleta.&nbsp;La fluorescencia no debe confundirse con la fosforescencia. En la fosforescencia, las part\u00edculas almacenan la energ\u00eda, irradi\u00e1ndola despu\u00e9s, hasta una vez que se ha finalizado la irradiaci\u00f3n de luz ultravioleta. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":32586,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-9431","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.1 (Yoast SEO v27.1.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Fluorescencia - Qu\u00e9 es y C\u00f3mo Funciona - Alquera.com<\/title>\n<meta name=\"description\" content=\"La fluorescencia es el fen\u00f3meno mediante el cual algunos productos o substancias emiten luz una vez se les irradia con luz ultravioleta.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/fluorescencia\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Fluorescencia - Qu\u00e9 es y C\u00f3mo Funciona\" \/>\n<meta property=\"og:description\" content=\"La fluorescencia es el fen\u00f3meno mediante el cual algunos productos o substancias emiten luz una vez se les irradia con luz ultravioleta.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.alquera.com\/en\/fluoresceina-sodica\/fluorescencia\/\" \/>\n<meta property=\"og:site_name\" content=\"Alquera.com\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/alqueraciencia\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-02T06:14:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.alquera.com\/wp-content\/uploads\/2020\/10\/fluorescencia2-300x171.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.alquera.com\/fluoresceina-sodica\/fluorescencia\/\",\"url\":\"https:\/\/www.alquera.com\/fluoresceina-sodica\/fluorescencia\/\",\"name\":\"Fluorescencia - 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